Identifying Potential Host Factor for SARS-Cov-2 Infection Using CRISPR Screening

In this study, we aimed to identify potential host factors for SARS-Cov-2 infection using CRISPR screening. We obtained nine genome-wide CRISPR knockout screen datasets from CRISP-view database which is analyzed by using MAGeCK. We found 272 genes that were significantly enriched or depleted in infected versus uninfected cells. Among these genes, eight were consistently identified as essential for coronavirus family infection across multiple studies, and five of them were identified as significant for SARS-Cov-2 infection, ACE2, CTSL, and TRAF3, RAB7A, SMARCA4. It is suggested that RAB7A, which is involved in the endosomal trafficking pathway, may play a critical role in SARS-CoV-2 infection, although this has not yet been published. We also found several genes that are potentially related with SARS-Cov-2 infection, including EMC1, TPT1, and LMAN2. Overall, our study highlights the potential of CRISPR screening to identify novel host factors and provides insights into the molecular mechanisms of SARS-Cov-2 infection.

Author

• Li, Junyan

Language

• en

Keyword

• host factors
• MAGeCK
• CRISP-view
• SARS-Cov-2
• CRISPR

Date created

• 2023

Type of Work

• Master's Thesis

License

• All rights reserved

Rights statement

• In Copyright

GW Unit

• Bioinformatics and Molecular Biochemistry

Degree

• M.S.

Advisor

• Li, Wei WL

Committee Member(s)

• Dimri, Manjari MD
• Lee, Jiyoung JL
• Li, Rong RL
• Mazumder, Raja RM
• Vanderhoek, Jack JV
• Weglicki, William WW
• Horvath, Anelia AH
• Gupta, Mamta MG
• Shook, Brett BS

Persistent URL

•  https://scholarspace.library.gwu.edu/etd/sn009z62f