Photonic and Ablation-based Ionization for the Analysis of Single Cells and Organelles Open Access
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A systemic understanding of the dynamic cellular processes in living organisms requires highly sensitive techniques that can rapidly detect and identify diverse biomolecules. Owing to the specificity and sensitivity of mass spectrometric analysis, it has become an indispensable tool for chemical analysis. The metabolic analysis of single cells and subcellular organelles is an emerging field that aims at studying cellular heterogeneity and its effect on the response of an organism to disease and environmental influences. Technical advances in mass spectrometry are required to make significant strides in this field. This dissertation describes my achievements based on photonic nanostructures and mid-infrared laser ablation for the mass spectral analysis of single cells and organelles.
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